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M94A0628.TXT
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1994-10-21
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Document 0628
DOCN M94A0628
TI Double-stranded strong-stop DNA and HIV reverse transcription.
DT 9412
AU Li P; Stephenson A; Kuiper L; Burrell CJ; National Centre for HIV
Virology Research, Institute of Medical; and Veterinary Science,
Adelaide, South Australia.
SO Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:70 (abstract no. FB7).
Unique Identifier : AIDSLINE ASHM5/94349027
AB Using a synchronized, one-step HIV infection model, we report here the
detection in acutely infected cells of a novel double-stranded
strong-stop HIV DNA with a discrete length of approximately 650 base
pairs, commencing at or near the left hand end of the right hand U3
region of the HIV long terminal repeat. The plus-strand of this
double-stranded strong-stop DNA possesses the primer binding site
sequence and appears to be synthesized prior to the completion of the
synthesis of its complementary minus-strand. In contrast, the
minus-strand of the double-stranded strong-stop DNA lacks the primer
binding site sequence after RNaseH digestion. We propose that a
transient free plus-strand strong-stop DNA is released from its template
by displacement synthesis and subsequently used as template for the
synthesis of its complementary minus-strand. The proposed transient free
plus-strand strong-stop DNA may also mediate the second template switch.
A revised model for HIV reverse transcription will be presented.
DE Base Composition/GENETICS Cell Line DNA, Viral/*GENETICS Human HIV
Infections/*MICROBIOLOGY Models, Genetic Repetitive Sequences, Nucleic
Acid/GENETICS Reverse Transcriptase/*GENETICS Virus
Integration/*GENETICS Virus Replication/*GENETICS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).